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KMID : 0670820090140010009
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Korean Journal of Medical Mycology
2009 Volume.14 No. 1 p.9 ~ p.15
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Protective Effects of Immunization with Proteinase, Phospholipase and Cell Wall Proteins of Candida albicans in Mice Candidosis
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Shin Woon-Seob
Yang Young-Chul
Choi Sun-Ju
Park Su-Jung
Park Joo-Young
Kim Soo-Kie
Lee Kyoung-Ho
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Abstract
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Background: The opportunistic fungus Candida albicans is a major pathogen especially to immunocompromised patients.
Objectives: We examined the protective effect of the active and passive immunizations to evaluate the applicability for the treatment of candidosis in Candida-infected mice model.
Methods: Candida cell wall components were obtained by treatment of lyticase, proteinase K, and dithiothreitol. The proteinase was purified from the culture filtrates of C. albicans using a series of chromatographic steps consisting of DEAE-Sepharose FF, Sephacryl S-200 HR and size-exclusion high performance liquid chromatography. The phospholipase was purified from the culture supernatant of C. albicans with DEAE column chromatography, reverse phase column chromatography, revere phase HPLC and size-exclusion HPLC. Antibodies to cell wall protein components, proteinase and phospholipase were produced by immunization into mice of same strain.
Results: The mean survival times of active and passive immunized mice groups were longer than those of non-immunized groups.
Conclusion: These results showed that immunization with proteinase and its antibody were the most effective to prolong survival time in Candida-infected mice. [Kor J Med Mycol 2009; 14(1): 9-15]
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KEYWORD
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Candida albicans, Proteinase, Phospholipase, Immunization
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